Imaging calcium concentration dynamics in small neuronal compartments
Publication information:
Yasuda R, Nimchinsky E, Scheuss V, Pologruto T, Oertner T, Sabatini B, Svoboda K. Imaging calcium concentration dynamics in small neuronal compartments. Sci STKE. 2004;2004(219):pl5. doi:10.1126/stke.2192004pl5
Abstract
Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.